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double stranded rna mimetic poly i c  (InvivoGen)


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    InvivoGen double stranded rna mimetic poly i c
    Double Stranded Rna Mimetic Poly I C, supplied by InvivoGen, used in various techniques. Bioz Stars score: 97/100, based on 347 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/rna+mimetic+poly+i+c/Poly/pmc07362904-115-9-13
    Average 97 stars, based on 347 article reviews
    double stranded rna mimetic poly i c - by Bioz Stars, 2026-10
    97/100 stars

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    Related Articles

    Cell Culture:

    Article Title: Type I interferon and interferon-stimulated gene expression in oral epithelial cells.
    Article Snippet: .. OKF6/Tert‐1 cells were treated with either 1 μg/ml double‐stranded RNA mimetic poly(I:C) (Invivogen, tlrl‐picw) in cell culture media for up to 6 hr RNA of cells was isolated using the RNEasy Plus Mini kit (Qiagen, 74136) and subsequently converted to cDNA via re‐ verse transcription using the iScript Supermix (Bio‐Rad, 1708840). .. Quantitative PCR was performed on the cDNA using SsoAdvanced SYBR Green (Bio‐Rad, 172‐5274) in the CFX96‐C1000 Touch ther‐ mocycler (Bio‐Rad).

    Article Title: Type I Interferon and Interferon-stimulated Gene Expression in Oral Epithelial Cells
    Article Snippet: .. RT-qPCR OKF6/Tert-1 cells were treated with either 1 μg/ml double-stranded RNA mimetic poly(I:C) (Invivogen, tlrl-picw) in cell culture media for up to six hours. .. RNA of cells was isolated using the RNEasy Plus Mini kit (Qiagen, 74136) and subsequently converted to cDNA via reverse transcription using the iScript Supermix (Bio-Rad, 1708840). qPCR was performed on the cDNA using SsoAdvanced SYBR Green (Bio-Rad, 172–5274) in the CFX96-C1000 Touch thermocycler (Bio-Rad).

    Article Title: Type I Interferon and Interferon-stimulated Gene Expression in Oral Epithelial Cells
    Article Snippet: .. OKF6/Tert-1 cells were treated with either 1 μg/ml double-stranded RNA mimetic poly(I:C) (Invivogen, tlrl-picw) in cell culture media for up to six hours. .. RNA of cells was isolated using the RNEasy Plus Mini kit (Qiagen, 74136) and subsequently converted to cDNA via reverse transcription using the iScript Supermix (Bio-Rad, 1708840). qPCR was performed on the cDNA using SsoAdvanced SYBR Green (Bio-Rad, 172–5274) in the CFX96-C1000 Touch thermocycler (Bio-Rad).

    Isolation:

    Article Title: Type I interferon and interferon-stimulated gene expression in oral epithelial cells.
    Article Snippet: .. OKF6/Tert‐1 cells were treated with either 1 μg/ml double‐stranded RNA mimetic poly(I:C) (Invivogen, tlrl‐picw) in cell culture media for up to 6 hr RNA of cells was isolated using the RNEasy Plus Mini kit (Qiagen, 74136) and subsequently converted to cDNA via re‐ verse transcription using the iScript Supermix (Bio‐Rad, 1708840). .. Quantitative PCR was performed on the cDNA using SsoAdvanced SYBR Green (Bio‐Rad, 172‐5274) in the CFX96‐C1000 Touch ther‐ mocycler (Bio‐Rad).

    Quantitative RT-PCR:

    Article Title: Type I Interferon and Interferon-stimulated Gene Expression in Oral Epithelial Cells
    Article Snippet: .. RT-qPCR OKF6/Tert-1 cells were treated with either 1 μg/ml double-stranded RNA mimetic poly(I:C) (Invivogen, tlrl-picw) in cell culture media for up to six hours. .. RNA of cells was isolated using the RNEasy Plus Mini kit (Qiagen, 74136) and subsequently converted to cDNA via reverse transcription using the iScript Supermix (Bio-Rad, 1708840). qPCR was performed on the cDNA using SsoAdvanced SYBR Green (Bio-Rad, 172–5274) in the CFX96-C1000 Touch thermocycler (Bio-Rad).



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    double stranded rna mimetic poly i c  (InvivoGen)
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    InvivoGen double stranded rna mimetic poly i c
    Double Stranded Rna Mimetic Poly I C, supplied by InvivoGen, used in various techniques. Bioz Stars score: 97/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    rna mimetic poly i c  (InvivoGen)
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    OKF6/Tert-1 cells were treated with 1 μg/ml <t>poly(I:C)</t> for up to six hours and measured for changes in type I interferon expression by RT-qPCR. Bar graphs and error bars represent the means and standard errors of measurement (SEM) of three independent experiments, each with three biological replicates per condition. *p < 0.05, **p < 0.005, and ***p < 0.0005 compared to 0h, using a two-tailed, unpaired Student’s t-test. All data analysis was performed using GraphPad Prism version 8.2.0 for Windows, GraphPad Software, La Jolla California USA, www.graphpad.com.
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    OKF6/Tert-1 cells were treated with 1 μg/ml poly(I:C) for up to six hours and measured for changes in type I interferon expression by RT-qPCR. Bar graphs and error bars represent the means and standard errors of measurement (SEM) of three independent experiments, each with three biological replicates per condition. *p < 0.05, **p < 0.005, and ***p < 0.0005 compared to 0h, using a two-tailed, unpaired Student’s t-test. All data analysis was performed using GraphPad Prism version 8.2.0 for Windows, GraphPad Software, La Jolla California USA, www.graphpad.com.

    Journal: Molecular oral microbiology

    Article Title: Type I Interferon and Interferon-stimulated Gene Expression in Oral Epithelial Cells

    doi: 10.1111/omi.12270

    Figure Lengend Snippet: OKF6/Tert-1 cells were treated with 1 μg/ml poly(I:C) for up to six hours and measured for changes in type I interferon expression by RT-qPCR. Bar graphs and error bars represent the means and standard errors of measurement (SEM) of three independent experiments, each with three biological replicates per condition. *p < 0.05, **p < 0.005, and ***p < 0.0005 compared to 0h, using a two-tailed, unpaired Student’s t-test. All data analysis was performed using GraphPad Prism version 8.2.0 for Windows, GraphPad Software, La Jolla California USA, www.graphpad.com.

    Article Snippet: OKF6/Tert-1 cells were treated with either 1 μg/ml double-stranded RNA mimetic poly(I:C) (Invivogen, tlrl-picw) in cell culture media for up to six hours.

    Techniques: Expressing, Quantitative RT-PCR, Two Tailed Test, Software

    OKF6/Tert-1 cells were treated with 1 μg/ml poly(I:C) for up to six hours and measured for changes in interferon-stimulated gene (ISG) expression by RT-qPCR. Measured genes were divided into each ISG basal expression group, according to Table 2: Low (A), Medium (B), and High (C). Bar graphs and error bars represent the means and standard errors of measurement (SEM) of three independent experiments, each with three biological replicates per condition. *p < 0.05, **p < 0.005, and ***p < 0.0005 compared to 0h, using a two-tailed, unpaired Student’s t-test.

    Journal: Molecular oral microbiology

    Article Title: Type I Interferon and Interferon-stimulated Gene Expression in Oral Epithelial Cells

    doi: 10.1111/omi.12270

    Figure Lengend Snippet: OKF6/Tert-1 cells were treated with 1 μg/ml poly(I:C) for up to six hours and measured for changes in interferon-stimulated gene (ISG) expression by RT-qPCR. Measured genes were divided into each ISG basal expression group, according to Table 2: Low (A), Medium (B), and High (C). Bar graphs and error bars represent the means and standard errors of measurement (SEM) of three independent experiments, each with three biological replicates per condition. *p < 0.05, **p < 0.005, and ***p < 0.0005 compared to 0h, using a two-tailed, unpaired Student’s t-test.

    Article Snippet: OKF6/Tert-1 cells were treated with either 1 μg/ml double-stranded RNA mimetic poly(I:C) (Invivogen, tlrl-picw) in cell culture media for up to six hours.

    Techniques: Expressing, Quantitative RT-PCR, Two Tailed Test